Revisiting host identification of bacteriophage Enc34: from biochemical to molecular.

In our 2012 genome announcement (J Virol 86:11403-11404, 2012, https://doi.org/10.1128/JVI.01954-12), we initially identified the host bacterium of bacteriophage Enc34 as Enterobacter cancerogenus using biochemical tests. However, later in-house DNA sequencing revealed that the true host is a strain of Hafnia alvei. Capitalizing on our new DNA-sequencing capabilities, we also refined the genomic termini of Enc34, confirming a 60,496-bp genome with 12-nucleotide 5' cohesive ends. IMPORTANCE: Our correction reflects the evolving landscape of bacterial identification, where molecular methods have supplanted traditional biochemical tests. This case underscores the significance of revisiting past identifications, as seemingly known bacterial strains may yield unexpected discoveries, necessitating essential updates to the scientific record. Despite the host identity correction, our genome announcement retains importance as the first complete genome sequence of a Hafnia alvei bacteriophage.

Endocarditis infecciosa en válvula nativa mitral por Hafnia alvei / Infective endocarditis in native mitral valve by Hafnia alvei

We report the case of a 40-year-old male patient with no prior personal pathological history, who had a prolonged febrile syndrome without being associated with other important clinical features. He was diagnosed with subacute bacterial endocarditis in the native mitral valve by an atypical pathogen: Hafnia Alvei. After an exhaustive review of the bibliography of the last 20 years corresponding to the Cuban medical journals, we conclude that we are facing the first clinical case of this type published in Cuba(AU)

Se presenta el caso clínico de un paciente masculino de 40 años de edad, sin antecedentes patológicos personales, portador de un síndrome febril prolongado sin asociarse a otros elementos clínicos de importancia. Se le diagnosticó una endocarditis bacteriana subaguda en válvula mitral nativa por un germen patógeno atípico: Hafnia alvei. Tras realizar una exhaustiva revisión de la bibliografía de los últimos 20 años correspondiente a las revistas médicas cubanas, se concluye que estamos frente al primer caso clínico de este tipo publicado en Cuba(AU)

Endocarditis infecciosa en válvula nativa mitral por Hafnia alvei / Infective endocarditis in native mitral valve by Hafnia alvei

We report the case of a 40-year-old male patient with no prior personal pathological history, who had a prolonged febrile syndrome without being associated with other important clinical features. He was diagnosed with subacute bacterial endocarditis in the native mitral valve by an atypical pathogen: Hafnia Alvei. After an exhaustive review of the bibliography of the last 20 years corresponding to the Cuban medical journals, we conclude that we are facing the first clinical case of this type published in Cuba(AU)

Se presenta el caso clínico de un paciente masculino de 40 años de edad, sin antecedentes patológicos personales, portador de un síndrome febril prolongado sin asociarse a otros elementos clínicos de importancia. Se le diagnosticó una endocarditis bacteriana subaguda en válvula mitral nativa por un germen patógeno atípico: Hafnia alvei. Tras realizar una exhaustiva revisión de la bibliografía de los últimos 20 años correspondiente a las revistas médicas cubanas, se concluye que estamos frente al primer caso clínico de este tipo publicado en Cuba(AU)

Isolation of Hafnia species from kimchi.

Eight commercially packaged kimchi products were examined over 15 days of storage at 4 degrees to evaluate the occurrence of Hafnia alvei (H. alvei). Additionally, 7 saeujeot products, as a possible ingredient source, were analyzed to examine the bacteria's origin. Over the storage period, kimchi samples had decreasing pH levels, which stabilized at pH 4.2; acidity increased to 0.9+/-0.1%. Lactose-nonfermenting bacteria, which H. alvei belongs to, gradually reduced in numbers over the kimchi storage. However, the relative frequency of H. alvei to lactose-nonfermenting bacteria tended to increase. From the kimchi samples, 58 H. alvei-presumptive colonies were selected. Forty-three colonies turned out to be H. alvei and 15 colonies were identified as other strains or of uncertain identification when the API 20E system was used. From further test, 3 of the 43 colonies were H. alvei (-) against the phage test. Finally, H. alvei was isolated from saeujeot, indicating that this ingredient can be an originating source of H. alvei in kimchi.